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rapamycin mtor  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rapamycin mtor
    Rapamycin Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rapamycin+mtor/pm41937014-34-36-47
    Average 86 stars, based on 1 article reviews
    rapamycin mtor - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Bioprocessed black rice bran extract (Hemicellan) ameliorates sarcopenic obesity in palmitate-induced C2C12 myotubes and high-fat diet-fed C57BL/6N mice
    Article Snippet: The WelCount cell proliferation assay kit for cell viability measurement was purchased from Welgene (Seoul, Korea). .. The primary antibodies used for western blot analysis were as follows: β-actin (#4967), sirtuin 1 (SIRT1) (#2028s), AMP-activated protein kinase (AMPK) α (#2532), p-AMPK α (#2535), protein kinase B (Akt) (#4685), p-Akt (#9271), forkhead box O3a (FoxO3a) (#2497), mammalian target of rapamycin (mTOR) (#2972), p-mTOR (Ser2448, #2971), protein kinase C θ (PKCθ) (#13643), p-PKCθ (#9377), protein kinase C ζ (PKCζ) (#9368), p-PKCζ (#2060), peroxisome proliferator-activated receptor γ (PPARγ) (#2443), CCAAT/enhancer binding protein α (C/EBPα) (#2295), and fatty acid synthase (FAS) (#3180), which were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Additionally, the Muscle-specific RING finger protein 1 (MuRF1) (C-11) antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and the Atrogin-1 (AP2041) antibody was purchased from ECM Biosciences (Brooklyn, NY, USA).

    Binding Assay:

    Article Title: Bioprocessed black rice bran extract (Hemicellan) ameliorates sarcopenic obesity in palmitate-induced C2C12 myotubes and high-fat diet-fed C57BL/6N mice
    Article Snippet: The WelCount cell proliferation assay kit for cell viability measurement was purchased from Welgene (Seoul, Korea). .. The primary antibodies used for western blot analysis were as follows: β-actin (#4967), sirtuin 1 (SIRT1) (#2028s), AMP-activated protein kinase (AMPK) α (#2532), p-AMPK α (#2535), protein kinase B (Akt) (#4685), p-Akt (#9271), forkhead box O3a (FoxO3a) (#2497), mammalian target of rapamycin (mTOR) (#2972), p-mTOR (Ser2448, #2971), protein kinase C θ (PKCθ) (#13643), p-PKCθ (#9377), protein kinase C ζ (PKCζ) (#9368), p-PKCζ (#2060), peroxisome proliferator-activated receptor γ (PPARγ) (#2443), CCAAT/enhancer binding protein α (C/EBPα) (#2295), and fatty acid synthase (FAS) (#3180), which were purchased from Cell Signaling Technology (Danvers, MA, USA). .. Additionally, the Muscle-specific RING finger protein 1 (MuRF1) (C-11) antibody was purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and the Atrogin-1 (AP2041) antibody was purchased from ECM Biosciences (Brooklyn, NY, USA).

    Saline:

    Article Title: Preventive Effect of Standardized <i>Lespedeza cuneata</i> Extract on Dexamethasone-Induced Muscle Atrophy in C57BL/6N Mice
    Article Snippet: Muscle atrophy, characterized by a decline in muscle mass and function, results from an imbalance between protein synthesis and degradation.. This study explored the effects of standardized Lespedeza cuneata extract (LCE) on dexamethasone (DEX)-induced muscle atrophy.. The mice were orally administered LCE for 17 days.

    Incubation:

    Article Title: Preventive Effect of Standardized <i>Lespedeza cuneata</i> Extract on Dexamethasone-Induced Muscle Atrophy in C57BL/6N Mice
    Article Snippet: Muscle atrophy, characterized by a decline in muscle mass and function, results from an imbalance between protein synthesis and degradation.. This study explored the effects of standardized Lespedeza cuneata extract (LCE) on dexamethasone (DEX)-induced muscle atrophy.. The mice were orally administered LCE for 17 days.

    Article Title: AGK2 and nicotinamide riboside reveal SIRT2's protective role in a diabetic-dietary rat model of NASH via deacetylation of NLRP3, LKB1, and FOXO3a and restoration of AMPK-ACC/PGC-1α-Nrf2 signaling.
    Article Snippet: Obesogenic diets coupled with type-2 diabetes accelerate hepatic fat buildup, oxidative stress, and chronic inflammation, driving progression to non-alcoholic steatohepatitis (NASH).. Sirtuin 2 (SIRT2) has been implicated in both metabolic and inflammatory regulation, but its role in NASH remains unclear.. Here, we investigated the effects of pharmacological SIRT2 activation with nicotinamide riboside (NR) versus inhibition with AGK2 in a diabetic dietary (STZ–HFD) rat model of NASH.

    other:

    Article Title: An exopolysaccharide-rich fraction from Bifidobacterium animalis subsp. lactis MG741 ameliorates high-fat diet-induced MASLD by enhancing intestinal barrier function.
    Article Snippet: Primary antibodies against fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), fatty acid transport protein 2 (FATP2), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were from Abcam (Cambridge, MA, USA), while antibodies targeting stearoyl-CoA desaturase 1 (SCD1), mammalian target of rapamycin (mTOR), and carbohydrate response element-binding protein (ChREBP) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Pyrolysis Gas Chromatography:

    Article Title: AGK2 and nicotinamide riboside reveal SIRT2's protective role in a diabetic-dietary rat model of NASH via deacetylation of NLRP3, LKB1, and FOXO3a and restoration of AMPK-ACC/PGC-1α-Nrf2 signaling.
    Article Snippet: Obesogenic diets coupled with type-2 diabetes accelerate hepatic fat buildup, oxidative stress, and chronic inflammation, driving progression to non-alcoholic steatohepatitis (NASH).. Sirtuin 2 (SIRT2) has been implicated in both metabolic and inflammatory regulation, but its role in NASH remains unclear.. Here, we investigated the effects of pharmacological SIRT2 activation with nicotinamide riboside (NR) versus inhibition with AGK2 in a diabetic dietary (STZ–HFD) rat model of NASH.



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    rtPA regulates the PI3K-AKT-mTOR pathway in the ICH in vitro cell model. (A, B) The DEGs between the control group and hemin group associated with the PI3K/AKT pathway (KEGG: mmu04151) and mTOR pathway (KEGG: mmu04150) were screened, and the transcriptional levels of DEGs in each group are presented as heatmaps. (C–F) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). **** P < 0.0001, vs . control group; ## P < 0.01, #### P < 0.0001, vs . hemin group. (G–J) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). (K, L) Analysis of apoptosis-associated proteins of neurons ( n = 3 per group). (M–P) Analysis of autophagy-associated proteins of neurons ( n = 3 per group). Data are shown as mean ± SEM and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs. hemin group; & P < 0.05, && P < 0.01, &&& P < 0.001, &&&& P < 0.0001, vs . hemin + rtPA group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. hemin + rtPA + DMSO group. AKT: RAC-alpha serine/threonine-protein kinase; DEGs: differential expression genes; DMSO: dimethyl sulfoxide; ICH: intracerebral hemorrhage; KEGG: Kyoto Encyclopedia of Genes and Genomes; LC3: microtubule-associated proteins 1A/1B light chain 3B; LY294002: PI3K inhibitor; mTOR: mammalian target of <t>rapamycin;</t> p62: sequestosome-1/ubiquitin-binding protein p62; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.
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    rtPA regulates the PI3K-AKT-mTOR pathway in the ICH in vitro cell model. (A, B) The DEGs between the control group and hemin group associated with the PI3K/AKT pathway (KEGG: mmu04151) and mTOR pathway (KEGG: mmu04150) were screened, and the transcriptional levels of DEGs in each group are presented as heatmaps. (C–F) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). **** P < 0.0001, vs . control group; ## P < 0.01, #### P < 0.0001, vs . hemin group. (G–J) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). (K, L) Analysis of apoptosis-associated proteins of neurons ( n = 3 per group). (M–P) Analysis of autophagy-associated proteins of neurons ( n = 3 per group). Data are shown as mean ± SEM and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs. hemin group; & P < 0.05, && P < 0.01, &&& P < 0.001, &&&& P < 0.0001, vs . hemin + rtPA group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. hemin + rtPA + DMSO group. AKT: RAC-alpha serine/threonine-protein kinase; DEGs: differential expression genes; DMSO: dimethyl sulfoxide; ICH: intracerebral hemorrhage; KEGG: Kyoto Encyclopedia of Genes and Genomes; LC3: microtubule-associated proteins 1A/1B light chain 3B; LY294002: PI3K inhibitor; mTOR: mammalian target of <t>rapamycin;</t> p62: sequestosome-1/ubiquitin-binding protein p62; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.
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    rtPA regulates the PI3K-AKT-mTOR pathway in the ICH in vitro cell model. (A, B) The DEGs between the control group and hemin group associated with the PI3K/AKT pathway (KEGG: mmu04151) and mTOR pathway (KEGG: mmu04150) were screened, and the transcriptional levels of DEGs in each group are presented as heatmaps. (C–F) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). **** P < 0.0001, vs . control group; ## P < 0.01, #### P < 0.0001, vs . hemin group. (G–J) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). (K, L) Analysis of apoptosis-associated proteins of neurons ( n = 3 per group). (M–P) Analysis of autophagy-associated proteins of neurons ( n = 3 per group). Data are shown as mean ± SEM and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs. hemin group; & P < 0.05, && P < 0.01, &&& P < 0.001, &&&& P < 0.0001, vs . hemin + rtPA group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. hemin + rtPA + DMSO group. AKT: RAC-alpha serine/threonine-protein kinase; DEGs: differential expression genes; DMSO: dimethyl sulfoxide; ICH: intracerebral hemorrhage; KEGG: Kyoto Encyclopedia of Genes and Genomes; LC3: microtubule-associated proteins 1A/1B light chain 3B; LY294002: PI3K inhibitor; mTOR: mammalian target of <t>rapamycin;</t> p62: sequestosome-1/ubiquitin-binding protein p62; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.
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    Image Search Results


    rtPA regulates the PI3K-AKT-mTOR pathway in the ICH in vitro cell model. (A, B) The DEGs between the control group and hemin group associated with the PI3K/AKT pathway (KEGG: mmu04151) and mTOR pathway (KEGG: mmu04150) were screened, and the transcriptional levels of DEGs in each group are presented as heatmaps. (C–F) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). **** P < 0.0001, vs . control group; ## P < 0.01, #### P < 0.0001, vs . hemin group. (G–J) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). (K, L) Analysis of apoptosis-associated proteins of neurons ( n = 3 per group). (M–P) Analysis of autophagy-associated proteins of neurons ( n = 3 per group). Data are shown as mean ± SEM and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs. hemin group; & P < 0.05, && P < 0.01, &&& P < 0.001, &&&& P < 0.0001, vs . hemin + rtPA group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. hemin + rtPA + DMSO group. AKT: RAC-alpha serine/threonine-protein kinase; DEGs: differential expression genes; DMSO: dimethyl sulfoxide; ICH: intracerebral hemorrhage; KEGG: Kyoto Encyclopedia of Genes and Genomes; LC3: microtubule-associated proteins 1A/1B light chain 3B; LY294002: PI3K inhibitor; mTOR: mammalian target of rapamycin; p62: sequestosome-1/ubiquitin-binding protein p62; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.

    Journal: Neural Regeneration Research

    Article Title: Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway

    doi: 10.4103/NRR.NRR-D-23-01953

    Figure Lengend Snippet: rtPA regulates the PI3K-AKT-mTOR pathway in the ICH in vitro cell model. (A, B) The DEGs between the control group and hemin group associated with the PI3K/AKT pathway (KEGG: mmu04151) and mTOR pathway (KEGG: mmu04150) were screened, and the transcriptional levels of DEGs in each group are presented as heatmaps. (C–F) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). **** P < 0.0001, vs . control group; ## P < 0.01, #### P < 0.0001, vs . hemin group. (G–J) Analysis of PI3K, p-AKT, AKT, p-mTOR, and mTOR of neurons ( n = 3 per group). (K, L) Analysis of apoptosis-associated proteins of neurons ( n = 3 per group). (M–P) Analysis of autophagy-associated proteins of neurons ( n = 3 per group). Data are shown as mean ± SEM and were analyzed by one-way analysis of variance followed by Tukey’s post hoc test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, vs. hemin group; & P < 0.05, && P < 0.01, &&& P < 0.001, &&&& P < 0.0001, vs . hemin + rtPA group; # P < 0.05, ## P < 0.01, ### P < 0.001, vs. hemin + rtPA + DMSO group. AKT: RAC-alpha serine/threonine-protein kinase; DEGs: differential expression genes; DMSO: dimethyl sulfoxide; ICH: intracerebral hemorrhage; KEGG: Kyoto Encyclopedia of Genes and Genomes; LC3: microtubule-associated proteins 1A/1B light chain 3B; LY294002: PI3K inhibitor; mTOR: mammalian target of rapamycin; p62: sequestosome-1/ubiquitin-binding protein p62; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.

    Article Snippet: The following primary antibodies were used for analysis: bcl2 (rabbit, 1:1000, Proteintech, Cat# 12789-1-AP, RRID: AB_2227948), bax (rabbit, 1:1000, Proteintech, Cat# 50599-2-Ig, RRID: AB_2061561), coiled-coil myosin-like bcl2-interacting protein (beclin1; rabbit, 1:1000, Proteintech, Cat# 11306-1-AP, RRID: AB_2259061), sequestosome-1/ubiquitin-binding protein p62 (SQSTM1/p62; rabbit, 1:1000, Abclonal, Cat# A11250, RRID: AB_2758477), microtubule-associated proteins 1A/1B light chain 3B (LC3; rabbit, 1:1000, Abcam, Cat# ab48394, RRID: AB_881433), endoplasmic reticulum chaperone BiP (Grp78/BIP; mouse, 1:1000, Proteintech, Cat# 66574-1-Ig, RRID: AB_2881934), cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6; rabbit, 1:1000, Proteintech, Cat# 24169-1-AP, RRID: AB_2876891), PRKR-like endoplasmic reticulum kinase (PERK; rabbit, 1:1000, Cell Signaling Technology, Cat# 3192S, RRID: AB_2095847), phospho-PERK (rabbit, 1:1000, Cell Signaling Technology, Cat# 3179S, RRID: AB_2095853), eukaryotic translation initiation factor 2 subunit alpha (eIF2α; rabbit, 1:1000, Cell Signaling Technology, Cat# 9722S, RRID: AB_2230924), phospho-eIF2α (rabbit, 1:1000, Cell Signaling Technology, 9721S, RRID: AB_330951), phosphatidylinositol 3-kinase regulatory subunit alpha (PI3 kinase p85; rabbit, 1:1000, Cell Signaling Technology, Cat# 4257S, RRID: AB_659889), RAC-alpha serine/threonine-protein kinase (AKT; rabbit, 1:1000, Cell Signaling Technology, Cat# 4691S, RRID: AB_915783), phospho-AKT (rabbit, 1:1000, Cell Signaling Technology, Cat# 4060S, RRID: AB_2315049), mammalian target of rapamycin (mTOR; rabbit, 1:1000, Cell Signaling Technology, Cat# 2983S, RRID: AB_2105622), phospho-mTOR (rabbit, 1:1000, Cell Signaling Technology, Cat# 2971S, RRID: AB_330970), and β-actin (mouse, 1:1000, Proteintech, Cat# 66009-1-Ig, RRID: AB_2687938).

    Techniques: In Vitro, Control, Quantitative Proteomics, Ubiquitin Proteomics, Binding Assay, Recombinant

    The PI3K inhibitor LY294002 reverses the anti-ER stress effect of rtPA and the EGF domain of rtPA may mediate the PI3K/AKT pathway in the ICH in vitro cell model. (A–C) Analysis of ER stress–associated proteins ( n = 3 per group). (D) Confocal images and three-dimensional reconstruction of endoplasmic reticulum continuity of neurons by ER tracker after rtPA and PI3K inhibitor LY294002 treatment. Scale bars: 3 µm. (E) Immunofluorescence images of p-PERK (red, labeled by Cy3) in neurons after rtPA and PI3K inhibitor LY294002 treatment. Scale bars: 50 µm. (F–H) Analysis of PI3K p85 and p-AKT. Data are represented as mean ± SEM ( n = 3 per group). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . hemin group; & P < 0.05, && P < 0.01, vs . hemin + rtPA group; # P < 0.05, vs . hemin + rtPA + DMSO group (one-way analysis of variance followed by Tukey’s post hoc test). (I) Transmission electron microscopy images of cells after rtPA and rtPA domain inhibitor treatment. Scale bar: 100 µm. 3D: Three-dimensional; AKT: RAC-alpha serine/threonine-protein kinase; ATF6: cyclic AMP-dependent transcription factor ATF-6 alpha; DAPI: 4′,6-diamidino-2-phenylindole; EGF: epidermal growth factor; eIF2α: eukaryotic translation initiation factor 2 subunit alpha; ER: endoplasmic reticulum; LY294002: PI3K inhibitor; mTOR: mammalian target of rapamycin; PERK: PRKR-like endoplasmic reticulum kinase; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.

    Journal: Neural Regeneration Research

    Article Title: Recombinant tissue plasminogen activator protects neurons after intracerebral hemorrhage through activating the PI3K/AKT/mTOR pathway

    doi: 10.4103/NRR.NRR-D-23-01953

    Figure Lengend Snippet: The PI3K inhibitor LY294002 reverses the anti-ER stress effect of rtPA and the EGF domain of rtPA may mediate the PI3K/AKT pathway in the ICH in vitro cell model. (A–C) Analysis of ER stress–associated proteins ( n = 3 per group). (D) Confocal images and three-dimensional reconstruction of endoplasmic reticulum continuity of neurons by ER tracker after rtPA and PI3K inhibitor LY294002 treatment. Scale bars: 3 µm. (E) Immunofluorescence images of p-PERK (red, labeled by Cy3) in neurons after rtPA and PI3K inhibitor LY294002 treatment. Scale bars: 50 µm. (F–H) Analysis of PI3K p85 and p-AKT. Data are represented as mean ± SEM ( n = 3 per group). * P < 0.05, ** P < 0.01, *** P < 0.001, vs . hemin group; & P < 0.05, && P < 0.01, vs . hemin + rtPA group; # P < 0.05, vs . hemin + rtPA + DMSO group (one-way analysis of variance followed by Tukey’s post hoc test). (I) Transmission electron microscopy images of cells after rtPA and rtPA domain inhibitor treatment. Scale bar: 100 µm. 3D: Three-dimensional; AKT: RAC-alpha serine/threonine-protein kinase; ATF6: cyclic AMP-dependent transcription factor ATF-6 alpha; DAPI: 4′,6-diamidino-2-phenylindole; EGF: epidermal growth factor; eIF2α: eukaryotic translation initiation factor 2 subunit alpha; ER: endoplasmic reticulum; LY294002: PI3K inhibitor; mTOR: mammalian target of rapamycin; PERK: PRKR-like endoplasmic reticulum kinase; PI3K: phosphatidylinositol 3-kinase regulatory subunit alpha; rtPA: recombinant tissue plasminogen activator.

    Article Snippet: The following primary antibodies were used for analysis: bcl2 (rabbit, 1:1000, Proteintech, Cat# 12789-1-AP, RRID: AB_2227948), bax (rabbit, 1:1000, Proteintech, Cat# 50599-2-Ig, RRID: AB_2061561), coiled-coil myosin-like bcl2-interacting protein (beclin1; rabbit, 1:1000, Proteintech, Cat# 11306-1-AP, RRID: AB_2259061), sequestosome-1/ubiquitin-binding protein p62 (SQSTM1/p62; rabbit, 1:1000, Abclonal, Cat# A11250, RRID: AB_2758477), microtubule-associated proteins 1A/1B light chain 3B (LC3; rabbit, 1:1000, Abcam, Cat# ab48394, RRID: AB_881433), endoplasmic reticulum chaperone BiP (Grp78/BIP; mouse, 1:1000, Proteintech, Cat# 66574-1-Ig, RRID: AB_2881934), cyclic AMP-dependent transcription factor ATF-6 alpha (ATF6; rabbit, 1:1000, Proteintech, Cat# 24169-1-AP, RRID: AB_2876891), PRKR-like endoplasmic reticulum kinase (PERK; rabbit, 1:1000, Cell Signaling Technology, Cat# 3192S, RRID: AB_2095847), phospho-PERK (rabbit, 1:1000, Cell Signaling Technology, Cat# 3179S, RRID: AB_2095853), eukaryotic translation initiation factor 2 subunit alpha (eIF2α; rabbit, 1:1000, Cell Signaling Technology, Cat# 9722S, RRID: AB_2230924), phospho-eIF2α (rabbit, 1:1000, Cell Signaling Technology, 9721S, RRID: AB_330951), phosphatidylinositol 3-kinase regulatory subunit alpha (PI3 kinase p85; rabbit, 1:1000, Cell Signaling Technology, Cat# 4257S, RRID: AB_659889), RAC-alpha serine/threonine-protein kinase (AKT; rabbit, 1:1000, Cell Signaling Technology, Cat# 4691S, RRID: AB_915783), phospho-AKT (rabbit, 1:1000, Cell Signaling Technology, Cat# 4060S, RRID: AB_2315049), mammalian target of rapamycin (mTOR; rabbit, 1:1000, Cell Signaling Technology, Cat# 2983S, RRID: AB_2105622), phospho-mTOR (rabbit, 1:1000, Cell Signaling Technology, Cat# 2971S, RRID: AB_330970), and β-actin (mouse, 1:1000, Proteintech, Cat# 66009-1-Ig, RRID: AB_2687938).

    Techniques: In Vitro, Immunofluorescence, Labeling, Transmission Assay, Electron Microscopy, Recombinant